H2N2V1, Main, Exploration, bibRecord, 002E44

[Analysis of mutations in the genome of cold-adapted strains of influenza A virus using extended modification of polymerase chain restriction method].

Identifieur interne : 002E44 ( Main/Exploration ); précédent : 002E43; suivant : 002E45

[Analysis of mutations in the genome of cold-adapted strains of influenza A virus using extended modification of polymerase chain restriction method].

Auteurs : I V Kiseleva ; A I Klimov

Source :

Voprosy virusologii [ 0507-4088 ]

RBID : pubmed:12508679

Descripteurs français

KwdFr :
- Adaptation physiologique (génétique), Basse température, Cartographie de restriction, Génome viral, Génotype, Mutation, Réaction de polymérisation en chaîne (), Substitution d'acide aminé, Virus de la grippe A (génétique).
MESH :
- génétique : Adaptation physiologique, Virus de la grippe A.
- Basse température, Cartographie de restriction, Génome viral, Génotype, Mutation, Réaction de polymérisation en chaîne, Substitution d'acide aminé.

English descriptors

KwdEn :
- Adaptation, Physiological (genetics), Amino Acid Substitution, Cold Temperature, Genome, Viral, Genotype, Influenza A virus (genetics), Mutation, Polymerase Chain Reaction (methods), Restriction Mapping.
MESH :
- genetics : Adaptation, Physiological, Influenza A virus.
- methods : Polymerase Chain Reaction.
- Amino Acid Substitution, Cold Temperature, Genome, Viral, Genotype, Mutation, Restriction Mapping.

Abstract

Cold-adapted influenza viruses A/Leningrad/13 4/17/5 7 (H2N2) (Len/17) and A/Leningrad/I 34/4 7/57 (H2N2) (Len/47) are used in Russia to prepare live reassortant cold-adapted influenza vaccines (LIV) for adults and children, respectively. Comparison between the nucleotide sequences of the Len/17 strain and the initial wild-type strain A/Leningrad/13 4/5 7 (H2N2) revealed ten nucleotide substitutions (eight of them encoding). Four additional substitutions (three encoding) were found in the genome of the Len/47 virus. Gene segment restriction site (PCR-restriction) analysis was used for identification of the genotype of reassortant influenza viruses. Conventional methods of PCR-restriction analysis detect only five encoding nucleotides substitutions in the internal genes of the Len/17 and seven substitutions in the internal genes of the Len/47 virus. An extended modification of the PCR-restriction method detect all encoding mutations in the internal genes of the Len/17 and Len/47 viruses (eight and eleven encoding substitutions, respectively). This method is advantageous for genome composition analysis of reassortant influenza vaccine strains and for investigating the genetic stability of LIV during replication in vaccines.

PubMed: 12508679

Affiliations:

Le document en format XML

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<term>Restriction Mapping</term>
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<term>Cartographie de restriction</term>
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<term>Génotype</term>
<term>Mutation</term>
<term>Réaction de polymérisation en chaîne</term>
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<front><div type="abstract" xml:lang="en">Cold-adapted influenza viruses A/Leningrad/13 4/17/5 7 (H2N2) (Len/17) and A/Leningrad/I 34/4 7/57 (H2N2) (Len/47) are used in Russia to prepare live reassortant cold-adapted influenza vaccines (LIV) for adults and children, respectively. Comparison between the nucleotide sequences of the Len/17 strain and the initial wild-type strain A/Leningrad/13 4/5 7 (H2N2) revealed ten nucleotide substitutions (eight of them encoding). Four additional substitutions (three encoding) were found in the genome of the Len/47 virus. Gene segment restriction site (PCR-restriction) analysis was used for identification of the genotype of reassortant influenza viruses. Conventional methods of PCR-restriction analysis detect only five encoding nucleotides substitutions in the internal genes of the Len/17 and seven substitutions in the internal genes of the Len/47 virus. An extended modification of the PCR-restriction method detect all encoding mutations in the internal genes of the Len/17 and Len/47 viruses (eight and eleven encoding substitutions, respectively). This method is advantageous for genome composition analysis of reassortant influenza vaccine strains and for investigating the genetic stability of LIV during replication in vaccines.</div>
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Serveur d'exploration H2N2

[Analysis of mutations in the genome of cold-adapted strains of influenza A virus using extended modification of polymerase chain restriction method].

[Analysis of mutations in the genome of cold-adapted strains of influenza A virus using extended modification of polymerase chain restriction method].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration H2N2
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